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Dilution and Serial Dilution Calculator

Stock volume for a target dilution, and the steps for a serial series.

Stock concentration

Target concentration

Final volume needed (mL)

Serial dilution factor per step

How it works

C1V1 equals C2V2. For a serial dilution each step multiplies by the same factor, so the number of steps needed is the log of the total dilution over the log of the per-step factor.

Worth knowing

Below about 10 microlitres a single-step dilution is dominated by pipetting error. Use a serial series instead.
Mix at every step of a serial dilution. Carryover on the tip is the usual source of a bad curve.
C1V1 = C2V2 holds for any concentration unit as long as both sides use the same one.